Jae-Hwan Jin의 모든 글

JNO-WICD-02

JNO-WICD-02

Filter Holder for BX50 WI

(OLYMPUS  – 32BP775)

45㎜ holder for 32㎜ filter

 

  •  45㎜ 필터용 위치에 32㎜ 필터를 사용할 수 있도록 만든  홀더
  • 여러개의 필터를 중첩하여 사용 가능함.
  • BX50-WI (Olympus)  광학 유닛 업그레이드시에 JNO-DICD-01과 같이 사용되어 지며,  32BP775 설치시에 필요한 홀더임.
45㎜ holder for 32㎜  filter
Filter holder for transmitted light (OLYMPUS BX)

 

Lecture Application

현미경 이론 교육 신청

(교육내용 구성)

현미경의 구조 이해

대물렌즈의 적합성 및 스펙 확인

현미경의 기본 관찰법 (BF, DF, Phase, DIC… )

TEL :  02-3473-4188
Mobile : 010-6413-6235
E-mail : jhjin@jnoptic.com

 

Lecturer Profile

Profile of   JIN Jae-Hwan ( Instructor )

CEO of JNOpTIC co., ltd

1999년 10월4일

(주)택산상역 입사

2000년 06월05일 ~ 06월15일 ( OLYMPUS Japan in Tokyo)

Basic Knowledge of Microscope and Repair Training Course

BX50 etc Certification

 

2001년12월04일 ( OLYMPUS Japan in Tokyo)

Measuring Microscope Calibration License

Main Model: STM6

License ID Number: M029

2003년09월04일 ( OLYMPUS Japan in Tokyo)

Biological Confocal Microscope Setup & Service Training

Main Model: FluoView300 & FluoView500

2003년10월03일 ( OLYMPUS Japan in Tokyo)

Inverted Microscope  & Research Stereo Microscope Service Training

Main Model: IX71 / SZX9

 

2004년08월17일 ~ 8월20일 ( OLYMPUS Japan in Tokyo)

Confocal Microscope Setup & Service Training

Main Model:  FV1000

2004년 10월11 ~ 10월13일 ( OLYMPUS Japan in Tokyo)

Confocal Microscope Setup & Service Training

Main Model:  FV1000 M-COMB(Multi Combiner)

 

2005년06월20일 ~ 06월23일 ( OLYMPUS Japan in Tokyo)

Research System Microscope Service Training

 Main Model: AX70

2005년08월01일~08월02일 ( OLYMPUS Japan in Tokyo)

Polarizing Microscope Instruction Training

Main Model: BX51P

2005년12월19일 ~ 19일 ( OLYMPUS Japan in Tokyo)

ZDC Training for Maintenance &  Basic Knowledge of Confocal Microscope

2005년12월20일 ~ 22일 ( OLYMPUS Japan in Tokyo)

현미경의 성능을 끌어내는 Brushup Course (GA Academy)

 

2006년08월02일 ~ 08월04일 ( OLYMPUS Japan in Tokyo)

Confocal Laser Scanning Microscope for Industrial Market

D class License(for ols3000)

Main Model:  OLS 3000(Lext)

2006년10월11일 ~ 10월15일 ( OLYMPUS Japan in Tokyo)

Confocal Laser Scanning Microscope Training for Bio Maket

Maintenance Service Training

Main Mode :  M-COMB(Multi Combiner for FV1000)

2006년12월09일 IX Repair 교육

 

2007년05월07일 ~ 05월11일 ( OLYMPUS Japan in Tokyo)

Modify Training for Zero-Drift Compensation Unit of IX81

IX2-Cusominsing Training (Optic Port Modify)

Main Model: IX71 & IX81-ZDC

2007년09월03일 ~ 09월08일 ( OLYMPUS Japan in Tokyo)

Leicense D class Trainging

Main Model: OLYMPUS Bio Confocal Microscope  FV1000

 

2008년04월08일 ~ 04월10일 ( OLYMPUS Japan in INA_Nagano )

IV100 Setup Training

(in vivo fluorescence molecular imaging systems)

2008년07월22일 ~ 07월23일  ( Narishige Group in Tokyo,  Japan )

 Narishige Maintenance Training

2008년07월24일 ~ 07월28일 ( OLYMPUS Japan in Tokyo)

Research Inverted Microscope  maintenance Training

Main Model: IX81

2009년05월18일 ~ 5월22일 ( OLYMPUS Japan in Tokyo)

OLYMPUS Bio Confocal Microscope  License Training

 License Grade: FV1000 C class

FV1000 Service License

2009년06월23일 ~ 06월26일 ( OLYMPUS Japan in Tokyo)

Confocal  Laser Scanning  Microscope  License Training

License Grade: FV10i  C class

FV10i Service License

 

2009년 10월19일 ~ 10월23일 ( OLYMPUS Japan in Tokyo )

Multi Photon Laser Scanning MICROSCOPE Setup Training

Main Model: MPE C class

 

2010년08월03일 ~ 08월08일 ( OLYMPUS Japan in Tokyo )

Modify Training for Zero-Drift Compensation Unit of IX81

Main Model: IX81-ZDC2

IX81-ZDC2 training License

2011년 04월18일

Start a Business  – JNOpTIC co., LTD

2013년 04월4일

특허 출원: 현미경을 이용한 샘플의 높이 측정 방법

2014년 06월12일

특허 등록: 현미경을 이용한 샘플의높이 측정 방법

Patent: Height measurement method of sample by microscope
현미경을 이용한 샘플의 높이측정방법

JNO-DPTS

JNO-DPTS (Down load  Catalog  JNO-DPTS)

The Unit to observe fluorescence image  and IR DIC image  at the same time with Multi-Dual Port tube of  BX51-WI
(Made by JNOpTIC co.,ltd )

JNOPTIC Multi-Dual Port System
JNOPTIC Multi-Dual Port System

 

  1. Simultaneously Observation

jno-dpts_1-1

2-1. One Way((IR-DIC 775nm)

jno-dpts_3-1

2-2. One Way((Visible Spectrum)

jno-dpts_4-1

 

Simultaneously observation
– CA3 pyramidal neuron, IR-DIC & Alexa Fluor 488 (50 uM)

20160323_1(FullF6)_2 for wp

20160323_1(1) for wp
CA3 pyramidal neuron Alexa Fluor 488, IR-DIC (Simultaneous observations)
Figure courtesy by Dr. Sooyun Kim, Seoul National University
20151209_2_stim1(CA3 pyramidal neuron) for wp
CA3 pyramidal neuron
AcquCAM 23S & JNO-RNB filter with BX51WI (Download Catalog AcquCAM 23S)
20151209_2(CA3 pyramidal neuron) for wp
CA3 pyramidal neuron
AcquCAM 23S & JNO-RNB filter with BX51WI (Download Catalog AcquCAM 23S)

JNO-ARM

Advance Realtime Monitor. Easy-to-Use, Image Enhancement, etc

1. Information

ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.ARM01

2. Simple measurement tools

toolbar

  • Measurement Tools: Count, Distance, Angle, Area, etc.
  • Guide Lines: Cross Line, Rectangular Lattice, Circular Grid
  • Scale Panel on Live/Image Screen
  • Available measurement on LIVE image and saved image mode

3-1. Specialization for observation of fluorescence images

Improvement Function about Fluorescence Microscopy Image. (Used function: Auto Level, Low level, Pseudo Color, Merge Image)
ARM02
 Bottom on the left is merged image without improvement of image. And the bottom on the right is merged image after improvement of image. These images are shot by JNOPTIC AcquCAM Pro/G3 camera

3-2-1 Effect of high-contrast image with simple operation (Monochrome)

Improvement Function about Microscopy Image Monochrome. (Used function: Auto Level)arm04Effective improvement of image with simple operation. This image is shot by JNOPTIC Pro/S3 camera

3-2-2 Effect of high-contrast image with simple operation (Color)

Improvement Function about Microscopy Image. (Used function: Auto Level)arm05Effective improvement of image with simple operation. These images are shot by JNOPTIC AcquCAM Pro/G3 camera

3-3-1 Live Pseudo Color (for color camera)

When you get fluorescence images using color camera, if the unwanted channel was shown because of cross talk, you could remove this channel to take advantage of function of Live Pseudo.
Improvement Function about Fluorescence Microscopy Live Camera Image. (Used function: Live Pseudo Color)
arm06
 The extraction of wanted color information from color image (Remove BR on RGB source) These images are shot by JNOPTIC AcquCAM Pro/G3 camera

3-3-2 Live Pseudo Color (for Monochrome camera)

When you get fluorescence images using monochrome camera, you can raise efficiency of acquisition for fluorescence images to add similar false color to the color to be shown on the eyepiece in real time.
Improvement Function about Fluorescence Microscopy Live Monochrome Camera Image. (Used function: Live Pseudo Color)
arm08
 Improvement of usage environment for monochrome camera to add false color on the image. These images are shot by JNOPTIC AcquCAM Pro/S3 camera

4. JNO-MHU(Option Unit)

Available measuring height with additional unit, JNO-MHU
※ JNO-MHU is sold separately as optional unit.
jno-mhu1
  jno-mhu2
 Left: Focus on top of sample (Z-axis reset), Right: Focus on bottom of sample (Z-axis height measurement)
jno-mhu3
 < Measuring condition : over 20x objective, temp 20°C >
Responding Model Measurement value unit Measurable height Error of measuring 1000㎛ Recommended measuring height
CX 41 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
CKX 41 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
BX – FM 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
BX 51/53 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛
MX 51 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛
MX 61L/61 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛

5. Reporting to Excel

excel

 

 

OpTIC Eye (Material)

JNOPTIC Image Analysis Software OpTIC Eye Series

Catalog_OpTIC Eye

  1. OpTIC Eye (Material)

기본측정+오토카운팅(입도분석용)+금속조직분석용
– 형상측정(상분율측정)
: 두가지 이상의 색상을 추출하여 면적율 계산
– 그레인사이즈(Grain Size Analysis)
: ASTM E 1382에 의한 금속표면의 결정립도 산출
: 교차점 / 면적 / 수동면적 추출방식
– 구상화율 분석(Nodular-graphite analysis)
: ASTM, ISO, JIS 규격에 따라 분석이 가능
– 비금속 개재물(Non-Metallic Inclusion)
: 산화물, 규산물, 황화물, 내화물, 광재등 추출

 

영상분석에 의한 구상화율 측정 (nodular-graphite analysis)

주철분석 소프트웨어 모듈로 ASTM, JIS, KS 규격에 따라 구상흑연주철의 구상흑연에 대하여 현미경영상분석을 통해 구상화율을 측정하고 결과를 표시합니다.

20
구상화율 측정 모듈의 사용자 인터페이스

 

영상분석에 의한 결정도립 측정 (rain Size Analysis)

 

  • ASTM에 의거, 금속표면의 Grainsize(결정립크기) 및 ASTM Grain Size Number(결정립도)를 산출합니다.
  • ASTM 결정립도를산출하기 위하여 교차추출 및 면적추출방식중 택일하여 간단히 측정할 수 있습니다.
22
교차추출(Intercept)방식의 측정
23
면적추출(Planimetric)방식의 측정

 

 

 

 

 

 

 

 

OpTIC Eye (Auto)

JNOPTIC Image Analysis Software OpTIC Eye Series

Catalog_OpTIC Eye

  1. OpTIC Eye (Auto)

기본측정+보고서+오토카운팅(입도분석용)
– 셀이미지 자동측정(5단계 easy측정)
: 영상분할, 측정항목설정, 측정, 분류, 성적서기능
– 면적, 면적비, 반경, 직경등 15개 항목 자동측정

Auto Counting

영상을 자동 분석하는 기능입니다. 영상을 개체들(Objects)과  배경(Background)으로 분할(Segmentation)함 으로써 측정할 영역을 한번에 검출해내고, 면적 등을 자동측정 합니다. Auto Count는 위의 차례대로 측정이 이루어 집니다.

  • 영상분할(Segmentation)
  • 측정항목설정(Define Measure)
  • 측정(Measure)
  • 분류(Classification)
  • 성적서(Report)

 

측정 항목 설정(Define Measure)

1

  • 원하는 측정 항목 설정
  • 측정항목별 그림에 의한 상세설명
  • 구간 설정: 상한, 하한 적용가능

 

측정결과를 분석/분류 (Classification)

23

View Measurement를 통한 실시간 측정 항목 display 측정

크기별로 그룹화(Classification), 그룹별 색상화 가능

 

Report

4

측정결과를 4가지 형태의 Chart로 표시: Histogram, Line graph, Pie, Scatter-gram Excel 전송 가능

 

 

 

 

 

 

 

 

 

 

Company Profile

  1.  회사 소개
  • ㈜제이엔옵틱은 광학장비 취급 전문 업체로  OEM 광학카메라 및 기타 광학 액세서리, 올림푸스 금속, 생물 현미경 외 어플리케이션을 제작, 판매하고 있습니다. 특히 제이엔옵틱에서 자체 개발한 이미지 분석 소프트웨어 ARM과 AcquCAM OEM 카메라의 패키지 제품은 국내/외의 현미경 시장에서 가격 대비 매우 우수한 제품으로 평가를 받고 있습니다.
  • ㈜제이엔옵틱은 광학현미경에 장착하여 사용하는 높이측정용 단차측정장치 JNO-MHU를 개발하여 현재 특허권을 보유하고 있으며 컨포컬 레이저광학장치 등의 특수 장비의 개발 및 판매, modify 제품 관련한 컨설팅 등으로 입지를 넓혀가고 있습니다.
  • ㈜제이엔옵틱은 고객의 요구에 맞는 다양하고 전문적인 상품을 개발하여 지원해 드릴 수 있도록 최선을 다하며 고객의 소리에 늘 귀 기울일 수 있도록 노력하겠습니다.

 

2. 회사 연혁

2011

Establishment : JNOPTIC in Apr. 13, 2011

S/W Development : ARM (Image Analysis S/W Advanced Real-time Monitor)

Seller : JNOPTIC OEM camera in the microscope market

Register : trademark ‘AcquCAM’ for JNOPTIC OEM camera

Sales : JNOPTIC OEM cameras with ARM S/W to end-user & reseller

2012

Importer : Chroma filter (Mar. 3, 2012)

App. Development : JNO-MHU measuring height unit for microscope

Exporter : JNOPTIC OEM camera with ARM S/W abroad to Japan, China

2013

Conversion : corporate company JNOPTIC CO., LTD. in Jan. 11. 2013

Apply for patent applications : Apr. 04, 2013

Acquisition of patent : ‘Height measurement method of sample by microscope’ in Jun. 12, 2014

Register : JNOPTIC OEM cameras, ARM S/W, JNO-MHU on Public Procurement Service

 

3. 회사 위치

JNO-R2(B)

JNOPTIC Filter JNO-R2(B)

Download Catalog_JNO-R2(B)

 

Filter set for Cy5/AlexaFluor 647/Draq5

EX(nm) EM(nm)
649 670

 


Spectrum Comparison Fluor and JNO-R2(B)

 

Cy5 VS JNO-R2(B)
Cy5 VS JNO-R2(B)

 


JNO-R2(B) Spectrum

 

JNO-R2(B) Spectrum
Filters Type
 AT620/50x EX
AT655DC BS
AT690/50m EM

JNO-R2(B) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 


Best with these fluorochromes

 

  • Alexa Fluor 647™
  • Atto 647N
  • Cy5™
  • DiD
  • Draq5
  • DyLight 649
  • MitoTracker Deep Red 633/MeOH
  • Nile Blue
  • Quasar® 670
  • SYTO® 60
  • TO-PRO™-3

 


 

JNO-R1(B)

JNOPTIC Filter JNO-R1(B)

Download Catalog_JNO-R1(B)

 

Filter set for Texas Red/mCherry/AlexaFluor 594

EX(nm) EM(nm)
590 617

Spectrum Comparison Fluor and JNO-R1(B)

 

Alexa Fluor 594 VS JNO-R1(B)

 


JNO-R1(B) Spectrum

 

JNO-R1(B)
JNO-R1(B) Spectrum
Filters Type
AT560/40x EX
AT600DC BS
AT635/60m EM

JNO-R1(B) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 


Best with these fluorochromes

 

  • Alexa Fluor 568™
  • Alexa Fluor 594™
  • CAL Fluor® Red 610
  • Cy3.5™
  • DyLight 594
  • FusionRed
  • Killer Red
  • mCherry
  • MitoTracker Red/MeOH
  • mKate2
  • mRFP1
  • ReAsH-CCPGCC
  • Rhodamine Red™-X
  • ROX
  • Sulforhodamine 101
  • Texas Red®
  • Texas Red®-X
  • X-rhod-1/Ca2+

 


 

JNO-G(B)

JNOPTIC Filter JNO-G(B)

Download Catalog_JNO-G(B)

 

Filter set for TRITC/Cy3/TagRFP/AlexaFluor546

    • Main Fluorochromes : TRITC
EX(nm) EM(nm)
555 580

Spectrum Comparison Fluor and JNO-G(B)

 

TRITC VS JNO-G(B)
TRITC VS JNO-G(B)

 


JNO-G(B) Spectrum

 

JNO-G(B) Spectrum
Filters Type
AT540/25x EX
AT565DC BS
AT605/55m EM

 


JNO-G(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 


JNO-G(B) Image

 

Filter Set : JNO-G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-G(B)
Camera : AcquCAM 3G
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-G(B)
Camera : AcquCAM 5G
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC

 


Best with these fluorochromes

 

  • Alexa Fluor 546™
  • Alexa Fluor 555™
  • Atto 550
  • CAL Fluor® Red 590
  • Cy3™
  • DiI
  • DsRed
  • DyLight 549
  • MitoTracker Orange/MeOH
  • Resorufin
  • Rhod-2
  • TagRFP
  • TAMRA
  • tdTomato
  • Tetramethylrhodamine isothiocyanate
  • TRITC

 


 

JNO-B(B)

JNOPTIC Filter JNO-B(B)

Filter set for EGFP/FITC/Cy2/AlexaFluor488

Download Catalog_JNO-B(B)

 

    • Main Fluorochromes : FITC
 EX(nm) EM(nm)
490 525

 


Fluor VS JNO-B(B) Spectrum

 

FITC VS JNO-B(B)
FITC VS JNO-B(B)

 


JNO-B(B) Spectrum

 

JNO-B(B) Spectrum
Filters Type
AT480/30x EX
 AT505DC BS
AT535/40m EM

 


JNO-B(B) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 


JNO-B(B) Image

 

Filter Set : JNO-B(B)
Camera : AcquCAM 23GR2
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-B(B)
Camera : AcquCAM 3G
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-B(B)
Camera : AcquCAM 5G
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC

 


Best with these fluorochromes

 

  • Acridine Orange + DNA
  • Alexa Fluor 488™
  • Atto 488
  • Azami Green
  • BODIPY FL/pH7.2
  • Calcein
  • Calcium Green™-1
  • Cy2™
  • DiO
  • DyLight 488
  • EGFP
  • Emerald GFP
  • FAM
  • FITC
  • Fluo-4
  • GFP
  • LysoTracker Green/pH 5.2
  • MitoTracker Green FM/MeOH
  • mWasabi
  • Oregon Green™ 488
  • SYBR® Green I
  • SYTO 9/DNA
  • ZsGreen1

 

JNO-U(B)

JNOPTIC 형광필터 JNO-U(B)

Download Catalog_JNO-U(B)

 

Filter set for DAPI/Hoechst/AlexaFluor350

    • Main Fluorochromes : DAPI
 EX(nm) EM(nm)
359 461

 


Fluor VS JNO-U(B) Spectrum

 

DAPI VS JNO-U(B)
DAPI VS JNO-U(B)

JNO-U(B) Spectrum

 

JNO-U(B)
JNO-U(B) Spectrum
Filters Type
 AT375/28x EX
AT415DC BS
 AT460/50m EM

 


JNO-U(B) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 


JNO-U(B) Image

 

Filter Set : JNO-U(B)
Camera : AcquCAM 23GR2
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B)
Camera : AcquCAM 3G
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B)
Camera : AcquCAM 5G
C-mount adapter : 0.5x
Lense : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC

 


Best with these fluorochromes

 

  • Alexa Fluor 350™
  • Biosearch Blue
  • Coumarin
  • DAPI
  • DyLight 350
  • Hoechst 33258
  • LysoTracker Blue/MeOH

 


 

JNO-WI-3

X Y Mover & Bridge Stage

Description
Material   Aluminum
표면 처리   Black –Anodizing
Travel Range (mm)   X    ±12.5     Y    ±12.5
높이 (mm)   170  ~ 220
Tap Hole 규격   M6
Tap Hole 간격 (mm)   X    25    Y    25