FISH

Fluorescence In Situ Hybridization
– 형광동소보합법 –

Many factors influence the quality of FISH

1. Processing & storage2. Specimen3. Probes
4. Microscope5. Filters6. Light source
Many factors influence the quality of FISH
Inadequat Contrast Image for FISHGood Contrast Image for FISH
필터 선정시 고려 할 사항설 명
ContrastSignal / Noise ratio =
MORE CONTRAST
BrightnessSave time, increase throughput
and reduce eye strain with brighter images
Color SeparationDistinguish between colors
more easily and exclude unwanted colors
RegistrationEach filter set produces images that are aligned with images from other filter sets
형광 필터 선정시 고려할 포인트

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