CoolLED_filter_table

Fliter Table for CoolLED illuminations.

Single Band Sets pE-300 Series pE-4000 pE-340fura
DAPI 39000 SB (UV@365) 385 (Ch.A) 49028 49028
DAPI 49028 MB (UV@400)
CFP 49013 435 (Ch.A) 49001 49013
GFP/FITCS 49002 460 (Ch.B) 49002 49002
YFP 49023 500 (Ch.C) 49003 49023
TRITC/Cy3 49004 550 (Ch.C) 49004 49004
TxRed/mCherry 49008 550 (Ch.C) 49008 49008
Cy5 49006 635 (Ch.D) 49009 49006
Cy7/IrDye800 N/A 740 (Ch.D) 49007 N/A

 

Full Multi Band Sets pE-300 Series pE-4000 pE-340fura
DAPI/FITC/TRITC 69013v2 385/470/550
(Ch.A.B.C)
69013v2 69013v2****
DAPI/FITC/TxRED 69013v2 385/490/580
(Ch.A.B.C)
69015** 69013v2****
DAPI/FITC/TRITC/Cy5 89402* 385/460/550/635
(Ch.A.B.C.D)
89402 89402****
GFP/mCherry 59022 460/550(Ch.B.C) 59022 59022*****
CFP/YFP 59017*** 435/500(Ch.A.B) 59017 59017***
CFP/YFP/mCherry 69008*** 435/500/580
(Ch.A.B.C)
69008 69008*****
CFP/YFP/RFP/Cy7 N/A N/A 89403 N/A

 

Pinkel Sets pE-300ultra MB(UV@400) pE-4000 pE-340fura
Chroma LED Chroma Chroma
FURA-2 N/A N/A N/A 79001
FURA-2/GFP N/A N/A N/A 79004
FURA-2/TRITC N/A N/A N/A 79003
DAPI/FITC/TRITC 69302 405/470/580
(Ch.A.B.C)
69302 N/A
DAPI/FITC/TxRED 69302 405/470/580
(Ch.A.B.C)
69302 N/A
DAPI/FITC/TRITC/Cy5 N/A 405/470/550/635
(Ch.A.B.C.D)
89400 N/A
GFP/mCherry 59222 460/580/
(Ch.B.C)
59222 N/A
CFP/YFP 59217*** 435/500
(Ch.A.B)
59217 N/A
CFP/YFP/mCherry 69308*** 435/500/580
(Ch.A.B.C)
69308 N/A
* pE-300lite will allow vis. Of all 4. pE-300white & pE-300ultra will allow manual and software control of DAvsFIvsTR&Cy5
** Very specific to TxRed/mCherry/Red FISH and but not recomended for TRITC/Cy3 etc..
*** Only recommended for stronger YFP signals
**** All visable, only independent control of DAPI – others via single source in the pE-340fura
***** All visiable but via same source -so no switching or balancing