카테고리 보관물: Chroma

Special Case of Filter Recommendation

Special Case of Filter Recommendation

 

CASE 1

고객 요청 : 하기의 사양에 맞는 필터 추천 문의 (하기의 이미지 참고)

  1. DIC : 592nm, 800nm 반사 & 580nm 이하 투과                                      EM : 580nm 이하 투과
  2. DIC : 670nm, 800nm 반사 & 560nm 이하 투과                                      EM : 560nm 이하 투과
  3. DIC : 750nm, 800nm 반사 & 740nm 이하 투과                                      EM : 740nm 이하 투과

조명장치 : 700-1000 nm 레이저

현미경 필터 큐브 : 니콘 Ti

 

Chroma 추천 Filter Set :

  1. ZT488/594rpc-UF3 (BS),                                                                  ET535/70m (EM),                                                                                ET535/70m (EM),                                                                                                        Filter cube 91032 (Nikon Ti)                                                                                  * 2 pcs EM as needed for blocking 2p STED (laser 750, 800nm)
  2. T640spxr-UF3 (BS),                                                                                  ET595/60m-2p (EM),                                                                                                  Filter cube 91032 (Nikon Ti)
  3. ZT4ZT760sprdc-UF3 (BS),                                                                  ET685/80m (EM),                                                                                          ET685/80m (EM),                                                                                                        Filter cube 91032 (Nikon Ti)                                                                                  * 2 pcs EM as needed for blocking 2p STED (laser 750, 800nm)

 

CASE 2

고객 요청 : 하기의 사양에 맞는 필터 추천 문의 (하기의 이미지 참고)

  1. DIC : 570nm 이하는 반사 & 570nm 이상은 투과

 

Chroma 추천 Filter :

  1. T575lpxr (BS)                                                                                                          Type: BS                                                                                                                                Size : 26x38mm                                                                                                          Thickness : 3mm                                                                                                            R425-569, T578-861 nm

 

CASE 3

고객 요청 : 하기의 사양에 맞는 필터 추천 문의

  1. DIC 1ea : 600nm cutting (사이즈 : 25 x 36 x 1mm)                              EM 1ea  : 550~600nm detecting 1ea  (사이즈 : 25mmR)                      EM : 600nm~650nm detecting 1ea (사이즈 : 25mmR)

현미경 : Leica TCS SP8 STED 3X

조명장치 : 레이저

Chroma 추천 Filter :

  1. ET575/40m (EM)                                                                                                         ET625/30m (EM)                                                                                                            T600lpxr (BS)

 

CASE 4

고객 요청 : 하기의 사양에 맞는 필터 추천 문의

  1. DIC : 780nm 이하 투과 780nm 이상 반사

조명장치 : Two-photon beam

현미경 필터 큐브 : 니콘 Ti

Chroma 추천 Filter :

  1. ZT775sp-2p_3m (BS)                                                                                            Type: BS (Multiphoton shortpass laser dichroic)                                         Size : 25.5x36mm                                                                                                          Thickness : 3mm                                                                                                            Reflects 775-1200nm and transmits 400-735nm                                      Wavelength (CWL): 747nm

 

 

Contact us

Contact Us

 

확인하시고자 하는 시약 및 필터는 자주 사용이 되지 않아 필터의 정보가 아직 준비가 되어 있지 않습니다. 하기의 연락처로 직접 문의 주시면 상담 드릴 수 있도록 하겠습니다.

  • Tel : 02-3473-4188~9
  • E-mail : jhjin@jnoptic.com

상담을 위하여 몇 가지 사항을 체크하여 주시면 보다 빠르게 확인하여 추천을 드릴 수가 있습니다.

[ 필터 주문 시 확인 사항 ]

->하기의 사항 중 확인 가능하신 것만 체크하여 알려주시기 바랍니다.

  • 사용하고자 하는 형광시약 또는 EX / EM 파장대
  • 현미경 및 장비의 모델명
  • 조명장치의 종류 (ex : mercury, xenon, laser 등)

 

 

 

 

 

 

49307

JNOPTIC Filter 49037

Download Catalog_49037

49037 Spectrum

49037 Spectrum
Filters Type
ET740/40x EX
 T770lpxr BS
ET780lp EM

 

– Filter set for Li_Cor for IR dye 800

Excitation () Emission ()
782 805

Spectrum Comparison Fluor and 49037

AlexaFluor 790 VS 49037

 

Best with these fluorochromes

 

49037 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

49015

JNOPTIC Filter 49015

Download Catalog_49015

49015 Spectrum

Filters Type
ET605/50x EX
 T635lpxr BS
ET670/50m EM

 

– Filter set for Alexa Fluor 633

Excitation () Emission ()
632 647

 

Spectrum Comparison Fluor and 49015

AlexaFluor 633 VS 49015

 

Best with these fluorochromes

 

49015 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

49031

JNOPTIC Filter 49031

Download Catalog_49031

49301 Spectrum

Filters Type
ET569/25x EX
 T588lpxr BS
ET615/40m EM

 

– Filter set for Alexa Fluor 568, Cy3.5

Excitation () Emission ()
578 603

 

This is a narrow-band filter set intended for applications where multiple fluorochromes are present in a sample and spectral separation from neighboring fluorochromes is essential. The 49008 set will provide a brighter signal, but may provide less effective spectral separation.

 

Spectrum Comparison Fluor and 49031

AlexaFluor 568 VS 49301

 

Best with these fluorochromes

 

49031 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

49907

JNOPTIC Filter 49907

Download Catalog_49907

49907 Spectrum

Filters Type
ZET532/10x EX
 ZT532rdc BS
ET575/50m EM

 

– Filter set of 532nm Laser Bandpass Set for mKO, mOrange2

For use with lasers between 529-535

 

Excitation () Emission ()
531 554

 

Spectrum Comparison Fluor and 49907

AlexaFluor 532 VS 49907

 

49907 Spec

  • Typical Application(s) : Laser (Non-TIRF)
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

49014

JNOPTIC Filter 49014

Download Catalog_49014

49014 Spectrum

Filters Type
ET530/30x EX
 T550lpxr BS
ET575/40m EM

 

– Filter set for mKO/mOrange

Excitation () Emission ()
531 554

 

Spectrum Comparison Fluor and 49014

AlexaFluor 532 VS 49014

 

Best with these fluorochromes

 

49014 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

49906

JNOPTIC Filter 49906

Download Catalog_49906

49906 Spectrum

Filters Type
ZET514/10x EX
 ZT514rdc BS
ET575/50m EM

 

– Filter set of 514nm Laser Bandpass Set for mKO, mOrange2

For use with lasers between 511-517

Excitation () Emission ()
517 539

 

Spectrum Comparison Fluor and 49906

AlexaFluor 514 VS 49906

 

49906 Spec

  • Typical Application(s) : Laser (Non-TIRF)
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

 

49023

JNOPTIC Filter 49023

Download Catalog_49023

49023 Spectrum

Filters Type
ET500/40x EX
 T525lpxr BS
ET560/50m EM

 

– Filter set for AlexaFluor 514/ZsYellow/Eosin

Excitation () Emission ()
517 539

 

Spectrum Comparison Fluor and 49023

AlexaFluor 514 VS 49023

 

Best with these fluorochromes

 

49023 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

49910

JNOPTIC Filter 49910

Download Catalog_49910

49910 Spectrum

Filters Type
ZET561/10x EX
 ZT514rdc BS
ET630/70m EM

 

– Filter set of 561nm Laser Bandpass Set for AlexaFluor 568, AlexaFluor 594, Texas Red®, mCherry

For use with lasers between 557-563

Excitation () Emission ()
587 610

 

Spectrum Comparison Fluor and 49910

mCherry VS 49910

 

49910 Spec

  • Typical Application(s) : Laser (Non-TIRF)
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

49915

JNOPTIC Filter 49915

Download Catalog_49915

49915 Spectrum

Filters Type
ZET365/20x EX
 ZT365bcm BS
ET425lp EM

 

– Filter set of 355-375nm Laser Longpass Set for Uncaging and Ablation

Excitation () Emission ()
346 442

 

Spectrum Comparison Fluor and 49915

Alexa Fluor 350 VS 49915

 

49915 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26×38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

19004

JNOPTIC Filter 19004

Download Catalog_19004

19004 Spectrum

Filters Type
AT540/25x EX
AT565DC BS
AT575lp EM

 

– Filter set for TRITC/Cy3 Longpass

  • Main Fluorochromes : FITC
Excitation () Emission ()
555 580

 

Spectrum Comparison Fluor and 19004

TRITC VS 19004

 

  • R-phycoerythrin

 

19004 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

 

19002

JNOPTIC Filter 19002

Download Catalog_19002

19002 Spectrum

Filters Type
AT480/30x EX
 AT505DC BS
AT515lp EM

 

– Filter set for GFP/FITC Longpass

  • Main Fluorochromes : FITC
Excitation () Emission ()
490 525

 

Spectrum Comparison Fluor and 19002

FITC VS 19002

 

  • Acridine Orange + DNA and RNA
  • Auramine-rhodamine
  • JC-1 non-ratiometric

 

19002 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

19000

JNOPTIC Filter 19000

Download Catalog_19000

19000 Spectrum

Filters Type
AT375/28x EX
AT415DC BS
AT435lp EM

– Filter set for UV/DAPI Longpass

  • Main Fluorochromes : DAPI
Excitation () Emission ()
359 461

 

Spectrum Comparison Fluor and 19000

DAPI VS 19000

Best with these fluorochromes

  • Calcofluor White
  • DAPI

 

19000 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

39007

JNOPTIC Filter 39007

Download Catalog_39007

39007 Spectrum

Filters Type
 AT620/50x EX
AT655DC BS
AT690/50m EM

 

– Filter set for Cy5/AlexaFluor 647/Draq5

  • Main Fluorochromes : Cy5
Excitation () Emission ()
649 670

 

Spectrum Comparison Fluor and 39007

Cy5 VS 39007

Best with these fluorochromes

 

39007 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

39010

JNOPTIC Filter 39010

Download Catalog_39010

39010 Spectrum

Filters Type
AT560/40x EX
AT600DC BS
AT635/60m EM

 

– Filter set for Texas Red/mCherry/AlexaFluor 594

Excitation () Emission ()
590 617

 

Spectrum Comparison Fluor and 39010

AlexaFluor 594 VS JNO-R1(B)

 

Best with these fluorochromes

 

39010 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

39004

JNOPTIC Filter 39004

Download Catalog_39004

39004 Spectrum

Filters Type
AT540/25x EX
AT565DC BS
AT605/55m EM

 

– Filter set for TRITC/Cy3/TagRFP/AlexaFluor546

  • Main Fluorochromes : TRITC
Excitation () Emission ()
555 580

Spectrum Comparison Fluor and 39004

TRITC VS 39004

Best with these fluorochromes

 

39004 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

39002

JNOPTIC Filter 39002

Download Catalog_39002

39002 Spectrum

Filters Type
AT480/30x EX
 AT505DC BS
AT535/40m EM

 

– Filter set for EGFP/FITC/Cy2/AlexaFluor488

  • Main Fluorochromes : FITC
Excitation () Emission ()
490 525

Spectrum Comparison Fluor and 39002

FITC VS 39002

 

Best with these fluorochromes

 

39002 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

39000

JNOPTIC Filter 39000

Download Catalog_39000

39000 Spectrum

Filters Type
 AT375/28x EX
AT415DC BS
 AT460/50m EM

 

– Filter set for DAPI/Hoechst/AlexaFluor350

  • Main Fluorochromes : DAPI
Excitation () Emission ()
359 461

Spectrum Comparison Fluor and 39000

DAPI VS 39000

 

Best with these fluorochromes

 

39000 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

49003

JNOPTIC Filter 49003

Download Catalog_49003

49003 Spectrum

Filters Type
ET500/20x EX
 T515LP BS
ET535/30m EM

 

– Filter set for EYFP

  • Main Fluorochromes : YFP
Excitation () Emission ()
514 527

 

Spectrum Comparison Fluor and 49003

YFP VS 49003

 

Best with these fluorochromes

 

49003 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes