카테고리 보관물: Fluorochrome

mCherry

 

mCherry

 

Information

 

mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 and has an emission maximum at 583 .

mCherry는 형광 물질 (형광 단백질)로서 분자 및 세포 구성 요소에 태그를 부착 할 때 marker 로 사용됩니다. mCherry와 대다수의 적색 형광 단백질은 Discosoma 말미잘에서 분리된 단백질 drFP583 (일반적으로 DsRed라고 함)에서 유래한 반면, 녹색 범위의 다른 형광 단백질은 종종 해파리 GFP의 변종입니다. DsRed는 558 ㎚에서 최적으로 여기되고, 583 ㎚ 에서 최대 방출을 갖는 4 량체입니다. 

 


Spectrum

 

mCherry Spectrum

 

Excitation () Emission ()
587 610

 


Recommended Filter set for mCherry

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R1(B) 560/40
(540~580)
600 (LP) 635/60
(605~665)
Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)
600 (LP) 630/75
(592.5~667.5)
Wide Excitation band,
Bandpass Emission
49306 580/25
(567.5~592.5)
600 (LP) 625/30
(610~640)
Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49910 561/10
(556~565)
561 (LP) 630/70
(595~665)
For use with laser between 557~563

Narrow Excitation band,
Bandpass Emission

49911 594/10
(589~599)
594 (LP) 645/75
(607.5~682.5)
For use with laser between 591~596

Narrow Excitation band,
Bandpass Emission

 


BFP

 

BFP

 

Information

 

Fluorescent proteins, like EBFP, can be used as protein “tags” to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

 

EBFP와 같은 형광 단백질은 자극이나 전이를 위한 안정적인 marker 로서 또는 그들의 일시적인 발현의 하위 세포를 연구하기 위해 단백질 “태그” 로 사용될 수 있습니다.

Spectrum

 

BFP Spectrum

 

Excitation () Emission ()
385 448

Recommended Filter set for BFP

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49021 405/20
(395~415)
425 (LP) 460/50
(435~485)
Narrow Excitation band,
Bandpass Emission
49028 395/25
(382.5~407.5)
425 (LP) 460/50
(435~485)
Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49901 405/20
(395~415)
405 (LP) 460/50
(435~485)
For use with laser between 400~410

Narrow Excitation band,
Bandpass Emission


Cy5.5

 

Cy5.5

 

Information

 

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

Cyanine 5.5 (Cy5.5) 염료는 형광 이미징 및 기타 형광 기반 생화학 분석을 위해 생물 분자를 표지하는 데 사용되었습니다. 그들은 펩타이드, 단백질 및 올리고 제 등의 라벨링에 널리 사용됩니다. Cy5.5 염료는 가장 일반적인 적색 형광체의 한 유형입니다.

 


 

Spectrum

 

Cy5.5 Spectrum
Excitation () Emission (㎚)
675 695

 


Recommended Filter set for for Cy5.5

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49022 545/25
(532.5~557.5)
565 (LP) 605/70
(570~640)
Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49914 642/20
(632~652)
647 (LP) 705/72
(669~741)
For use with laser between 633~650

Narrow Excitation band,
Bandpass Emission

 


 

tdTomato

 

tdTomato

 

Information

 

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Its excitation and emission maxima occur at 554 and 581 , respectively. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

 

tdTomato는 특히 응집현상을 줄이도록 디자인된 tdTomato 유전자 2개의 유전적 융합체2) 로 이루어졌다. tdTomato의 tandem dimer 구 조는 형광단백질의 밝기에 중요한 영향을 미친 다. tdTomato의 excitation과 emission은 각각 554 과 581 ㎚ 에서 최고 값을 보인다.  tdTomato는 intramolecular dimer이기 때문에 monomer와 유사하게 작용하며 N-말단과 C-말 단의 융합에 성공적으로 이용된다. 이것은 뛰어 난 광안정성을 보이며 maturation(성숙) 반감기 (t0.5)는 37℃에서 1시간이다.

 


Spectrum

 

tdTomato Spectrum
Excitation () Emission ()
554 581

 


Recommended Filter set for tdTomato

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)
565 (LP) 605/55
(577.5~632.5)
Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)
565 (LP) 605/70
570~640)
Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)
565 (LP) 590/33
(573.5~606.5)
Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)
532 (LP) 590/50
(565~615)
For use with laser between 529~535

Narrow Excitation band,
Longpass Emission

49909 561/10
(556~566)
561 (LP) 600/50
(575~625)
For use with laser between 557~563

Narrow Excitation band,
Bandpass Emission

 

 


 

YFP

 

YFP

 

Information

 

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 and its emission peak is 527 . Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.

노란색 형광 단백질 (YFP)은 원래 해파리 Aequorea victoria에서 파생 된 녹색 형광 단백질 (GFP)의 유전 적 돌연변이입니다. 그것의 여기 피크는 514 이고 방출 피크는 527 입니다. 부모 GFP와 마찬가지로 YFP는 형광 현미경 검사에 유용한 그 특성 덕분에 세포 및 분자 생물학에서 유용한 도구입니다.

 


 

Spectrum

 

YFP Spectrum

 

EX() EM()
514 527

 


Recommended Filter set for YFP

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39003 495/20
(485~505)
515 (LP) 535/30
(520~550)
Narrow Excitation band,
Bandpass Emission
49003 500/20
(490~510)
515 (LP) 540/30
(525~555)
Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49905 514/10
(509~519)
514 (LP) 545/40
(525~565)
For use with laser between 511~517

Narrow Excitation band,
Bandpass Emission

 

 


 

GFP

 

GFP

 

Information

 

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 and a minor one at 475 . Its emission peak is at 509 , which is in the lower green portion of the visible spectrum.

녹색 형광 단백질 (GFP)은 238 아미노산 잔기 (26.9 kDa)로 구성된 단백질로 청색에서 자외선까지의 빛에 노출되면 밝은 녹색 형광을 나타냅니다. 많은 다른 해양 생물이 유사한 녹색 형광 단백질을 가지고 있지만, GFP는 전통적으로 해파리 Aequorea victoria에서 먼저 분리 된 단백질을 의미합니다. A. 빅토리아의 GFP는 파장 395 ㎚ 에서 주요한 여기 피크와 475 ㎚ 에서 사소한 피크를 나타내고 그 방출 피크는 가시 광선 스펙트럼의 낮은 녹색 부분에 있는 509 입니다.

 


Spectrum

 

GFP Spectrum
Excitation () Emission ()
488 507

 


Recommended Filter set for for GFP

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)
505 (LP) 535/40
(515~555)
Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)
495 (LP) 525/50
(500~550)
Wide Excitation band,
Bandpass Emission
49020 480/20
(470~490)
495 (LP) 510/20
(500~530)
Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)
488 (LP) 525/50
(500~550)
For use with laser between 485~491

Narrow Excitation band,
Bandpass Emission

 

 


 

EtBr

 

EtBr

 

Information

 

As with most fluorescent compounds, ethidium bromide is aromatic. Its core heterocyclic moiety is generically known as a phenanthridine, an isomer of which is the fluorescent dye acridine. Absorption maxima of EtBr in aqueous solution are at 210 and 285 , which correspond to ultraviolet light. As a result of this excitation, EtBr emits orange light with wavelength 605 .

대부분의 형광 화합물과 마찬가지로 에티듐 브로마이드는 방향족으로  중심 헤테로시 클릭 잔기는 페난 트리 딘으로 일반적으로 알려져 있으며, 이의 이성질체는 형광 염료 아 크리 딘입니다. 수용액에서의 EtBr의 흡수 최대 값은 210 ㎚ 와 285 ㎚ 에서 자외선에 해당하며 이 여기의 결과로 EtBr은 파장 605 ㎚ 의 주황색 광을 방출합니다.

 


Spectrum

 

EtBr Spectrum
Excitation () Emission ()
520 603

 


Recommended Filter set for for EtBr

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39009 480/30
(465~495)
505 (LP) 620/60
(590~650)
Medium Excitation band,
Bandpass Emission
49005 545/30
(530~560)
570 (LP) 620/60
(590~650)
Medium Excitation band,
Bandpass Emission

  • 레이저 Lighting source -> Please contact us.

 

 


 

 

Cy7

 

Cy7

 

Information

 

Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.

Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.

시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.

Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.

Cy7은 육안으로 보이지 않는 근적외선 (near-IR) 불소로서 (여기 / 방출 최대 750/776 ) Cy7.5 염료뿐만 아니라 생체 내 이미징 응용 프로그램에 사용됩니다.

Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.

 


Spectrum

 

Cy7 Spectrum
Excitation () Emission ()
743 767

 


Recommended Filter set for for Cy7

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49007 710/75
(672.5~747.5)
760 (LP) 810/90
(765~855)
Very Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> Please contact us.

 

 

 


 

CFP

 

CFP

 

Information

 

Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 laser line and is optimally detected at 485 .

시안 형광 단백질 (Cyan Fluorescent Protein, CFP)은 생리 학적 과정을 모니터링하고, 단백질의 위치를 시각화하며, 생체 내에서 유전자 변형 (transgenic expression)을 검출하는 다목적 생물학적 표지자로서 CFP는 405 nm 레이저 라인에 의해 여기 될 수 있으며 485 에서 최적으로 검출됩니다.

 


Spectrum

 

CFP Spectrum
Excitation () Emission ()
433 475

 


Recommended Filter set for for CFP

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39001 435/20
(425~445)
455 (LP) 480/30
(465~495)
Narrow Excitation band,
Bandpass Emission
49001 436/20
(426~446)
455 (LP) 480/40
(460~500)
Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49902 436/20
(426~446)
442 (LP) 480/40
(460~500)
For use with laser between 430-445

Narrow Excitation band,
Bandpass Emission

49903 436/20
(426~4460)
442 (LP) 500/40
(480~520)
For use with laser between 430-445

Narrow Excitation band,
Bandpass Emission

 


 

Cy5

 

Cy5

 

Information

 

Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 laser lines, and visualized Cy5 filter sets.  In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.

Cy5 염료는 633 또는 647 레이저 라인을 사용하여 여기되고 Cy5 필터 세트를 시각화 할 수있는 밝은 원색 – 형광 염료로서 면역 세포 화학 응용뿐만 아니라 핵산 표지에도 일반적으로 사용됩니다.

 


Spectrum

 

Cy5 Spectrum
Excitation () Emission ()
649 670

 


Recommended Filter set for for Cy5

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R2(B) 620/50
(595~645)
655 (LP) 690/50
(665~715)
Wide Excitation band,
Bandpass Emission
49006 620/60
(590~650)
660 (LP) 700/75
(662.5~737.5)
Very Wide Excitation band,
Bandpass Emission
49009 640/30
(625~655)
660 (LP) 690/50
(665~715)
Medium Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49913 635/20
(625~645)
640 (LP) 655(LP) For use with laser between 627~643

Narrow Excitation band,
Longpass Emission

49914 642/20
(632~652)
647 (LP) 705/72
(669~741)
For use with laser between 633~650

Narrow Excitation band,
Bandpass Emission