tdTomato

 

tdTomato

 

Information

 

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Its excitation and emission maxima occur at 554 and 581 , respectively. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

 

tdTomato는 특히 응집현상을 줄이도록 디자인된 tdTomato 유전자 2개의 유전적 융합체2) 로 이루어졌다. tdTomato의 tandem dimer 구 조는 형광단백질의 밝기에 중요한 영향을 미친 다. tdTomato의 excitation과 emission은 각각 554 과 581 ㎚ 에서 최고 값을 보인다.  tdTomato는 intramolecular dimer이기 때문에 monomer와 유사하게 작용하며 N-말단과 C-말 단의 융합에 성공적으로 이용된다. 이것은 뛰어 난 광안정성을 보이며 maturation(성숙) 반감기 (t0.5)는 37℃에서 1시간이다.

 


Spectrum

 

tdTomato Spectrum
Excitation () Emission ()
554 581

 


Recommended Filter set for tdTomato

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)
565 (LP) 605/55
(577.5~632.5)
Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)
565 (LP) 605/70
570~640)
Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)
565 (LP) 590/33
(573.5~606.5)
Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)
532 (LP) 590/50
(565~615)
For use with laser between 529~535

Narrow Excitation band,
Longpass Emission

49909 561/10
(556~566)
561 (LP) 600/50
(575~625)
For use with laser between 557~563

Narrow Excitation band,
Bandpass Emission