Compare filter sets by selecting the recommended set listed for widefield sets and laser sets.
- If you need more information, please click Chroma filter medel No. You can see each fluorochrome information and recommended filter sets.
- If you need other information or looking for other model No., please contact us.
- Tel : 02-3473-4188~9
- E-mail : firstname.lastname@example.org
Filter Set Guide
This list provides information about the filter sets Chroma Technology has created for the multitude of fluorochromes that
are typically used in epi-fluorescence microscopy. We are aware that there are applications which require filter combinations
that are not listed. Often we can create such sets out of the large collection of special-order filters and dichroic beamsplitters
that we maintain in inventory. Occasionally we have to manufacture newly designed filters and/or mirrors. We understand the
limitations of the research budget and in either situation we try to keep prices within reasonable limits.
Please contact us for help in creating filter combinations that you do not find in the catalog.
Additionally, Chroma manufactures filters for a variety of instruments including gene chip readers, high-throughput systems,
plate readers, flow cytometers, confocal and multi-photon microscopes, Raman spectroscopy and single-molecule microscopy.
Often the manufacturers of these instruments provide specifications for the filters that they recommend. Sometimes you may wish to order filters that are different from their recommendations and sometimes the manufacturers of bioassays have their own recommendations.
Please contact us for custom sets and recommended filter sets for standard bioassays.
How to lead filters
Excitation filter D480/30x—The center wavelength is at 480 nm; full bandwidth is 30 nm [ = ±15]. In some cases when the bandwidth is not specified, the letter “x” is used to define the filter as an excitation filter. This is generally used for narrow band UV excitation filters, e.g. D340x.
Dichroic beamsplitter 505DCLP—The cut-on wavelength (50% transmission point) is approximately 505 nm for this longpass dichroic design. Dichroic mirrors may be named for the 50% point, but the actual reflection and transmission wavelengths are what really define the mirror. Therefore, a mirror that is called a 505DCLP may vary somewhat in the 50% transmission point. Feel confident that we will design the mirrors, regardless of name, to reflect and transmit the necessary bands. It would be much more accurate to name the mirrors for the reflection/transmission regions, but this is very cumbersome and we have not figured out the best way to accomplish this just yet.
Emission filter D535/40m—The center wavelength is at 535 nm; full bandwidth is 40 nm [ = ±20].
LP—longpass filter, which transmits wavelengths longer than the cut-on and blocks shorter wavelengths
SP—shortpass filter, which transmits wavelengths shorter than the cuton and blocks longer wavelengths
DCXR—dichroic longpass, extended reflection
DCXRU—dichroic longpass, extended reflection including the UV
PC—polychroic beamsplitter, reflects and transmits more than two
bands of ligh
ET—indicates highest throughput sputter coated filter or set
AT—indicates high throughput sputter coated filter or set
How to order filters
Answers to the following questions are extremely helpful when discussing the appropriate filters or filter sets for your use:
1. Which fluorochrome(s) is (are) being used?
2. What is the wavelength range of excitation and emission for those fluorochromes with which we are not familiar?
3. What is the make and model of the microscope or instrument being used?
4. What is the size of the excitation and/or emission filters if a filter wheel is being used in either the excitation or emission
5. Does the filter need to be image quality or not? This is particularly important for absorption-type emission filters.
6. What is the maximum thickness possible in the filter mount to be used?
7. What type of detector is being used (e.g. PMT, CCD, film)?
8. What are the specific reflection and transmission requirements for the dichroic beamsplitter?
Dichroic beamsplitters are designed with various spectral profiles. No dichroic beamsplitter reflects all wavelengths short of the
cut-on wavelengths, nor can it transmit all wavelengths beyond that point. Being as specific as possible will increase your
chances of getting the beamsplitter that you require and will diminish the possibility of paying too much for a beamsplitter that
is more than you require. It is best if we know the excitation and emission filters being used. This information is particularly
important when ordering a dichroic beamsplitter in the absence of any other filters.