GFP

 

GFP

 

Information

 

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 and a minor one at 475 . Its emission peak is at 509 , which is in the lower green portion of the visible spectrum.

녹색 형광 단백질 (GFP)은 238 아미노산 잔기 (26.9 kDa)로 구성된 단백질로 청색에서 자외선까지의 빛에 노출되면 밝은 녹색 형광을 나타냅니다. 많은 다른 해양 생물이 유사한 녹색 형광 단백질을 가지고 있지만, GFP는 전통적으로 해파리 Aequorea victoria에서 먼저 분리 된 단백질을 의미합니다. A. 빅토리아의 GFP는 파장 395 ㎚ 에서 주요한 여기 피크와 475 ㎚ 에서 사소한 피크를 나타내고 그 방출 피크는 가시 광선 스펙트럼의 낮은 녹색 부분에 있는 509 입니다.

 


Spectrum

 

GFP Spectrum
Excitation () Emission ()
488 507

 


Recommended Filter set for for GFP

 

If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

 

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)
505 (LP) 535/40
(515~555)
Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)
495 (LP) 525/50
(500~550)
Wide Excitation band,
Bandpass Emission
49020 480/20
(470~490)
495 (LP) 510/20
(500~530)
Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)
488 (LP) 525/50
(500~550)
For use with laser between 485~491

Narrow Excitation band,
Bandpass Emission