Overview of Typical Filter Sets
Schematic of Typical Filter Cube
The schematic of a typical filter cube for an inverted microscope is illustrated like below.
Multi Band Filter Set
One filter configuration is a set including a multi band exciter, multi band dichroic, and multi band emission filter. Each filter can be created to selectively transmit and block multiple bands of wavelengths.
This configuration requires the user to use a color CCD camera and simultaneously capture multiple fluorochromes present in a sample with a single image.
- Bandpass filters are designed to transmit a specific range of wavelengths and block light on either side of that range by their center wavelength and bandwidth.
- LP and SP filters are denoted by their cut-on or cut-off wavelengths at 50% of peak transmission. LP or SP filters that have a very sharp slope.
- Neutral Density (ND) filter blocks all wavelengths of light evenly.
This image is a schematic diagram of a typical fluorescence microscope, which uses incident-light illumination. This is the most common type of fluorescence microscope. Its most important feature is that by illuminating
with incident light it need only filter out excitation light scattering back from the specimen or reflecting from glass surfaces.
Over the years Chroma has accumulated many images from the scientists that use Chroma filters in their research.