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MX63/MX63L

현미경 솔루션

 

MX63 / MX63L

 

Streamline Your Large Sample Inspection Workflow

The MX63 and MX63L microscope systems are optimized for high-quality inspections of wafers as large as 300 mm, flat panel displays, circuit boards, and other large samples. Their modular design enables you to choose the components you need to tailor the system to your application.

These ergonomic and user-friendly microscopes help increase throughput while keeping inspectors comfortable while they do their work. Combined with OLYMPUS Stream image analysis software, your entire workflow, from observation to report creation, can be simplified.

 


Functional

 

Leading-Edge Analysis Tools

The MX63 series’ versatile observation capabilities provide clear, sharp images so users can reliably detect defects in their samples. New illumination techniques and image acquisition options within OLYMPUS Stream image analysis software give users more choices for evaluating their samples and documenting their findings.


The Invisible Becomes Visible: MIX Observation and acquisition

MIX observation technology produces unique observation images by combining darkfield with another observation method, such as brightfield, fluorescence, or polarization. MIX observation enables users to view defects that are difficult to see with conventional microscopes. The circular LED illuminator used for darkfield observation has a directional darkfield function where only one quadrant is illuminated at a given time. This reduces a sample’s halation and is useful for visualizing a sample’s surface texture.

Structure on semiconductor wafer

Condenser


Easily Create Panoramic Images: Instant MIA

With multiple image alignment (MIA), users can stitch images together quickly and easily simply by moving the KY knobs on the manual stage—a motorized stage is not necessary. OLYMPUS Stream software uses pattern recognition to generate a panoramic image, giving users a wider field of view.

Instant MIA image of a coin


Create all-in-focus images: EFI

The Extended Focus Imaging (EFI) function within OLYMPUS Stream captures images of samples whose height extends beyond the depth of focus of the objective and stacks them together to create one image that is all in focus. EFI can be executed with either a manual or motorized Z-axis and creates a height map for easy structure visualization. It is also possible to construct an EFI image while offline within Stream Desktop.

Stud bump on an IC chip


Capture Both Bright and Dark Areas Using HDR

Using advanced image processing, high dynamic range (HDR) adjusts for differences in brightness within an image to reduce glare. HDR improves the visual quality of digital images thereby helping to generate professional-looking reports.


From Basic Measurement to Advanced Analysis

Measurement is essential to quality and process control and inspection. With this in mind, even the entry-level OLYMPUS Stream software package includes a full menu of interactive measurement functions, with all measurement results saved with image files for further documentation. In addition, the OLYMPUS Stream Materials Solution offers an intuitive, workflow-oriented interface for complex image analysis. At the click of a button, image analysis tasks can be executed quickly and precisely. With a significant reduction in processing time for repeated tasks, operators can concentrate on the inspection at hand.


Efficient Report Creation

Creating a report can often take longer than capturing the image and taking the measurements. OLYMPUS Stream software provides intuitive report creation to repeatedly produce smart and sophisticated reports based on pre-defined templates. Editing is simple and reports can be exported to Microsoft Word or PowerPoint software. In addition, OLYMPUS Stream software’s reporting function enables digital zooming and magnification on acquired images. Report files are a reasonable size for easier data exchange by email.


Stand-Alone Camera Option

Using a DP22 or DP27 microscope camera, the MX63 series becomes an advanced stand-alone system. The cameras can be controlled via a compact box that requires only minimal space, helping users maximize their laboratory space while still capturing clear images and making basic measurements.


Advanced Designed to Support Cleanroom Conformity

The MX63 series is designed to work in a cleanroom and has features that help minimize the risk of contaminating or damaging samples. The system has an ergonomic design that helps keep users comfortable, even during prolonged use. The MX63 series complies with international specifications and standards, including SEMI S2/S8, CE, and UL.

Optional Wafer Loader Integration ― AL120 System*

An optional wafer loader can be attached to MX63 series to safely transfer both silicon and compound semiconductor wafers from a cassette to the microscope stage without using tweezers or wands. Renowned performance and reliability enable safe, efficient front and back macro inspections while the loader helps improve productivity in the laboratory.

MX63 combined with the AL120 wafer loader (200 mm version)

 


Fast, Clean Inspections

The MX63 series delivers contamination-free wafer inspections. All motorized components are housed in a shielded structure, and antistatic processing is applied to the microscope frame, tubes, breath shield, and other parts. The rotation speed of the motorized nosepieces is faster and safer than manual nosepieces, decreasing the time between inspections while keeping the operator’s hands below the wafer, reducing potential contamination.


System Design Achieving Efficient Observations

The XY stage is capable of both coarse and fine stage movements thanks to the combination of a built-in clutch and the XY knobs. The stage helps make observations efficient, even for large samples, such as 300 mm wafers.
The tilting observation tube’s extensive range enables operators to sit at the microscope in a comfortable posture.


System Design Achieving Efficient Observations

The XY stage is capable of both coarse and fine stage movements thanks to the combination of a built-in clutch and the XY knobs. The stage helps make observations efficient, even for large samples, such as 300 mm wafers.
The tilting observation tube’s extensive range enables operators to sit at the microscope in a comfortable posture.


Accepts All Wafer Sizes

Wafer holders and glass plates

The system works with various types of 150–200 mm and 200–300 mm wafer holders and glass plates. Should the size of the wafters change on the production line, the microscope’s frame can be modified at minimal cost. With the MX63 series, different stages can be used to accommodate 75 mm, 100 mm, 125 mm, and 150 mm wafers on the inspection line.


 

PE-4000

 

CoolLED pE-4000

 

Overview

NEW GENERATION LEDs The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy.  The system has 16 selectable LED wavelengths across 4 channels that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available.

The CoolLED pE-4000 now benefits from our award winning sustainable Greentechnology and delivers enhanced intensity at your microscope sample plane with a significant reduction in energy consumption, and is supplied with a 3 year warranty after registration.

 

The NEW Enhanced pE-4000 is a powerful 4-channel high-specification LED illumination system. It is flexible, controllable and environmentally friendly.  Benefits include:

SELECTABILITY

  • Choice of 256 wavelength combinations from 16 installed LEDs (365nm -770nm)
  • Choose 1 of 4 wavelengths for each 4 channels on the control pod
  • Unique wavelength grouping concept (see below)
  • Optimum wavelength to suit your experiment
  • Specific wavelength characterisation for optimal optogenetic control of cell responses

Wavelength Grouping Concept

As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains.  These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

 

DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available.  This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

 

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets.  The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

 

The pE-4000 allows multiple experiments with varying wavelengths on the same sample , as shown here.

(A) Representative photocurrent traces of a PhobosCA expressing CA1 cell evoked with different activation wavelengths and shutoff with 595nm light

(B) Photocurrent traces in the same cell evoked with 460nm light and shutoff with indicated wavelengths (10mW/mm2)

Wietek et al (2017) Anion-conducting channelrhodopsins with tuned spectra and modified kinetics engineered for optogenetic manipulation of behaviour,  Scientific Reports volume 7, Article number 14957(2017) doi: 10.1038/s41598-017-14330-y

 

SUSTAINABILITY

  • Lowest Power Consumption – other LED technology uses 120-350 Watts
  • >25000 hours operation
  • Highly stable, repeatable LED technology
  • Market leading energy efficiency

 

CELL VIABILITY

  • Optimal illumination through control features to extend fluorescence of cells
  • Reduced photobleaching and phototoxicity
  • Microsecond switching
  • Variable pulse duration on/off

“When you can only control intensity of ‘white’ light (rather than individual channels), the level of photobleaching can be high.  With the pE-4000, we can control the excitation of the individual channels.  It is possible to optimise the excitation intensity according to the labelling, greatly reducing photobleaching and phototoxicity in a live experiment.”    Dr Yan Gu, University of Sussex

“Suddenly, we were able to offer users uninterrupted extended live cell experiments of 100+ hours, without worrying about brightness fluctuations, lamps burning, room heating, etc. Also, users have reported markedly reduced bleaching and phototoxicity in their samples, both from the prokaryotic and the eukaryotic research fields.”     Dr Jens Eriksson, Oslo University Hospital

 

CONTROLLABILITY

  • Advance integration into major microscope software platforms
  • Save wavelength combinations and return to previous set-up
  • USB interface
  • High speed TTL Triggering
  • Analogue control – dynamic intensity sinusoidal control option
  • White light control
  • Expansion Box
  • Individual channel intensity control
  • Pre-determined intensity saving option

 

VISIBILITY

  • High signal to noise ratio which gives cleaner images and data

“Striking, bright fluorescence images with a strong signal to noise ratio even when using a low magnification objective” 

Graham Wright, Institute of Medical Biology, A*STAR Singapore

Time series of a confluent sheet of keratinocytes migrating during a live-cell imaging outgrowth assay, acquired on an Olympus IX-83 microscope equipped with a CoolLED pE-4000 lightsource (Fig. 4). Scale bar = 150 μm

 

ADAPTABILITY

  • Fits to all major research and clinical microscopes
  • Delivers light through a liquid light guide or fiber
  • Offers inline removable excitation filter holders
  • Uses the Universal Collimator

 

RELIABILITY

  • Assured through experience and QA assured processes
  • 3 year Warranty after registration
  • First year Warranty swap

The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics.  A dual mode control pod gives manual control in either White or Advanced modes.  Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber.  LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.

 

 

 

VF-900-0Z Vibratome

VF-900-0Z

 

The VF-900-0Z is designed for sectioning the tissue of larger animals. With a diameter of up to 165 mm, it allows the sectioning of human brains.  Many neuroanatomy and neuropathology experiments require sectioning entire brains from these animals. The VF-900-0Z is capable of sectioning slices for these tissues down to 40 microns.
With our patented compression technology, this fully automated slicer excels at sectioning slices used for:

The Compresstome® is also easy to use. Our online training videos will allow everyone in your lab to produce excellent slices.

 

How is the Compresstome® different from a vibratome?  You can read about a discussion thread on ResearchGate on selecting the best tissue sectioning tool for you.

The Compresstome® is also the best machine to cut adult and senior brain slices.  The paper and the associated protocol can be found in Brain Slice Methods.

 

 

Products we recommend to purchase with VF-900-0Z:

To reduce contamination when working with both fresh and fixed tissue we recommend purchasing additional:

Consumables we recommend for quality use include:

 

Down load  Technical Specification_VF-900-0Z

 

VF-800-0Z Vibratome

VF-800-0Z

 

The VF-800-0Z is designed for sectioning the tissue of larger animals.  With a diameter of up to 95 mm, it allows the brain sectioning of cat, dogs, pigs, and monkeys.  Many neuroanatomy and neuropathology experiments require sectioning entire brains from these animals. The VF-800-0Z is capable of sectioning slices for these large primates down to 40 microns.
With our patented compression technology, this fully automated slicer excels at sectioning slices used for:

How is the Compresstome® different from a vibratome?  You can read about a discussion thread on ResearchGate on selecting the best tissue sectioning tool for you.

The Compresstome® is also the best machine to cut adult and senior brain slices.  The paper and the associated protocol can be found in Brain Slice Methods.

Products we recommend to purchase with VF-800-0Z:

To reduce contamination when working with both fresh and fixed tissue we recommend purchasing additional:

To reduce contamination when working with both fresh and fixed tissue we recommend purchasing additional:

Consumables we recommend for quality use include:

 

Down load  Technical Specification_VF-800-0Z

 

VF-700-0Z Vibratome

VF-700-0Z

 

The VF-700-0Z is designed specifically for sectioning fixed tissues.  With a wider diameter of 21 mm, it can accommodate both rat and mice brains.  With our patented compression technology, this fully automated slicer excels at sectioning slices used for:

Our slicer technology allows you to double your slice viability.  The Compresstome® is also easy to use. Our online training videos will allow everyone in your lab to produce excellent slices.

 

 

Products we recommend to purchase with VF-700-0Z:

To reduce contamination when working with both fresh and fixed tissue we recommend purchasing additional:

Consumables we recommend for quality use include:

 

Down load  Technical Specification_VF-700-0Z

 

 

ACQUCAM 23GR IMAGES

AcquCAM 23GR

 

More images with AcquCAM 23GR

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L), B(L), G(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : PlanApo60xWLSM
Above three images are merged and processed using ARM S/W

 

 

AcquCAM 23GR

AcquCAM 23GR

 

(Down load Catalog  – ACQUCAM 23GR)

 

  • USB 3.0 Color Camera
  • 1/1.2 ” Exmor Sensor
  • 1920×1200 pixel
  • Trigger input and I/O
  • Available  of taking images with a wavelength of 650nm or more, such as CY5
  • High Sensitivity Sensor (Best)
  • Fast, seamless image transfer
  • Available of images with a very wide field of view
  • Low Noise Sensor

 

 

The displays live digital images with gradual smoothness and combines exceptional resolution  with faithful color reproduction.The new sensors feature a global shutter function and able to capture a high-speed moving image without focal plane distortion. High-speed processing, low noise and low power dissipation by using column-parallel A/D conversion. equipped with trigger mode, and the external pulse can control accumulation time. The Sensor also have a pulse output function to indicate respective conditions during shutter operation and can be coordinated with peripheral circuits. High-definition images can be displayed live at a rate of high frames per second. without compression. Such imaging quality enables even the finest cellular regions to be observed clearly and distinctly without deterioration. While focusing is made stress free.

Images with AcquCAM 23GR

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

 

JNO-ARM

Advance Realtime Monitor. Easy-to-Use, Image Enhancement, etc

 

1. Information

ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.

 

2. Simple measurement tools

  • Measurement Tools: Count, Distance, Angle, Area, etc.
  • Guide Lines: Cross Line, Rectangular Lattice, Circular Grid
  • Scale Panel on Live/Image Screen
  • Available measurement on LIVE image and saved image mode

 

3-1. Specialization for observation of fluorescence images

Improvement Function about Fluorescence Microscopy Image. (Used function: Auto Level, Low level, Pseudo Color, Merge Image)

Bottom on the left is merged image without improvement of image. And the bottom on the right is merged image after improvement of image. These images are shot by JNOPTIC AcquCAM Pro/G3 camera

 

3-2-1 Effect of high-contrast image with simple operation (Monochrome)

Improvement Function about Microscopy Image Monochrome. (Used function: Auto Level)

Effective improvement of image with simple operation. This image is shot by JNOPTIC Pro/S3 camera

 

3-2-2 Effect of high-contrast image with simple operation (Color)

Improvement Function about Microscopy Image. (Used function: Auto Level)

Effective improvement of image with simple operation. These images are shot by JNOPTIC AcquCAM Pro/G3 camera

 

3-3-1 Live Pseudo Color (for color camera)

When you get fluorescence images using color camera, if the unwanted channel was shown because of cross talk, you could remove this channel to take advantage of function of Live Pseudo.
Improvement Function about Fluorescence Microscopy Live Camera Image. (Used function: Live Pseudo Color)
 The extraction of wanted color information from color image (Remove BR on RGB source) These images are shot by JNOPTIC AcquCAM Pro/G3 camera

3-3-2 Live Pseudo Color (for Monochrome camera)

When you get fluorescence images using monochrome camera, you can raise efficiency of acquisition for fluorescence images to add similar false color to the color to be shown on the eyepiece in real time.
Improvement Function about Fluorescence Microscopy Live Monochrome Camera Image. (Used function: Live Pseudo Color)
 Improvement of usage environment for monochrome camera to add false color on the image. These images are shot by JNOPTIC AcquCAM Pro/S3 camera

4. JNO-MHU(Option Unit)

Available measuring height with additional unit, JNO-MHU

※ JNO-MHU is sold separately as optional unit.

Left: Focus on top of sample (Z-axis reset), Right: Focus on bottom of sample (Z-axis height measurement)

 < Measuring condition : over 20x objective, temp 20°C >

Responding Model Measurement value unit Measurable height Error of measuring 1000㎛ Recommended measuring height
CX 41 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
CKX 41 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
BX – FM 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
BX 51/53 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛
MX 51 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛
MX 61L/61 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛

 

5. Reporting to Excel

 

Specification

Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats& Frame rate 1920×1200 RGB24@ 54 fps
1920×1200 Y800@ 162 fps
1920×1200 Y16@ 80 fps                             1920×1200 Y411 @ 108 fps
1920×1200 YUY2 @ 81 fps
Dynamic range 8 / 12 bit
Exposure time 20 ㎲ to 30s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65g

 

 

More images with AcquCAM 23GR

-> For more details, click here.

 

 

 

AcquCAM 23S2 Images

AcquCAM 23S2

 

More images with AcquCAM 23S2

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L), B(L), G(L)
Camera : AcquCAM 23S2
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23S2
C-mount adapter : 1X
Lens : PlanApo60xWLSM
Above three images are merged and processed using ARM S/W

 

 

AcquCAM 23S2

AcquCAM 23S2

 

(Down load Catalog – ACQUCAM 23S2)

 

  • USB 3.0 Monochrome Camera
  • 1/1.2 ” Pregius Sensor
  • 1920×1200 pixel
  • Trigger input and I/O
  • Cost-effective mono camera for fluorescence microscope (Best)
  • Cost-effective camera for Department of Physiology (Best)
  • 1/1.2 inch optimized sensor size for microscope field of view
  • Acquire images of optimum width combined with 0.63x adapter

 

 

AcquCAM 23S2 mono camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The mono camera ships with the very sensitive 1/1.2 inch CMOS Pregius  sensor. With up to 162images per second, the AcuqCAM 23S2 is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses. Using the optional CS to M12 board lens adapter, the camera is
also compatible to M12 board lenses.

 

 

Images with AcquCAM 23S2

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23S2
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

 

Specification

Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats

& Frame rate

1920×1200 Y16 @ 80 fps

1920×1200 Y800 @ 162 fps

Sensitivity 0.015 lx
Exposure time 20㎲ to 30 s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65 g

 

ARM

ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.

-> For more details, click here.

 

 

More images with AcquCAM 23S2

-> For more details, click here.

AcquCAM 3G Images

AcquCAM 3G

 

More images with AcquCAM 3G

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : PlanApo60xWLSM
Above three images are merged and processed using ARM S/W

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L), B(L), G(L)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

AcquCAM 3G

AcquCAM 3G

 

(Down load Catalog – ACQUCAM 3G)

 

  • USB 3.0 Color Camera
  • 1/1.8 ” Pregius Sensor
  • 2048 x 1536 pixel
  • Trigger input and I/O
  • Cost-effective color camera
  • High Sensitivity Sensor
  • Fast, seamless image transfer
  • Available of images with a very wide field of view
  • Low Noise Sensor

 

AcquCAM 3G color camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The color camera ships with the very sensitive 1/1.8 inch CMOS Pregius  sensor. With up to 60 images per second, the AcuqCAM 3G is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses. Using the optional CS to M12 board lens adapter, the camera is
also compatible to M12 board lenses.

 

 

Images with AcquCAM 3G

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

 

Specification

Spec Description
Size 1/1.8 inch
Resolution H: 2048, V: 1536
Pixel size H: 3.45 µm, V: 3.45 µm
Video formats& Frame rate 2048×1536 (3.1 MP) RGB64 @ 60 fps

2048×1536 (3.1 MP) RGB32 @ 60 fps
2048×1536 (3.1 MP) Y16 @ 60 fps

2048×1536 (3.1 MP) Y411 @ 60 fps
2048×1536 (3.1 MP) YUY2 @ 60 fps

Dynamic range 8 / 12 bit
Exposure time 20 ㎲ to 30s
Lens mount C/Cs mount
Interface USB 2.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65g

 

 

ARM

ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.

-> For more details, click here.

 

 

More images with AcquCAM 3G

-> For more details, click here.

AcquCAM 5G Images

AcquCAM 5G

 

More images with AcquCAM 5G

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 5G
C-mount adapter : 1X
Lens : PlanApo60xWLSM
Above three images are merged and processed using ARM S/W

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L), B(L), G(L)
Camera : AcquCAM 5G
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

 

AcquCAM 23GR2 Images

AcquCAM 23GR2

 

More images with AcquCAM 23GR2

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L), B(L), G(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : PlanApo60xWLSM
Above three images are merged and processed using ARM S/W

 

 

 

 

 

AcquCAM 23GR2

AcquCAM 23GR2

 

(Down load Catalog  – ACQUCAM 23GR2)

 

  • USB 3.0 Color Camera
  • 1/1.2 ” Exmor Sensor
  • 1920×1200 pixel
  • Trigger input and I/O
  • Available  of taking images with a wavelength of 650nm or more, such as CY5
  • High Sensitivity Sensor (Best)
  • Fast, seamless image transfer
  • Available of images with a very wide field of view
  • Low Noise Sensor

 

The displays live digital images with gradual smoothness and combines exceptional resolution  with faithful color reproduction.The new sensors feature a global shutter function and able to capture a high-speed moving image without focal plane distortion. High-speed processing, low noise and low power dissipation by using column-parallel A/D conversion. equipped with trigger mode, and the external pulse can control accumulation time. The Sensor also have a pulse output function to indicate respective conditions during shutter operation and can be coordinated with peripheral circuits. High-definition images can be displayed live at a rate of high frames per second. without compression. Such imaging quality enables even the finest cellular regions to be observed clearly and distinctly without deterioration. While focusing is made stress free.

 

 

Images with AcquCAM 23GR2

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

JNO-ARM

Advance Realtime Monitor. Easy-to-Use, Image Enhancement, etc

 

1. Information

ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.

 

2. Simple measurement tools

  • Measurement Tools: Count, Distance, Angle, Area, etc.
  • Guide Lines: Cross Line, Rectangular Lattice, Circular Grid
  • Scale Panel on Live/Image Screen
  • Available measurement on LIVE image and saved image mode

 

3-1. Specialization for observation of fluorescence images

Improvement Function about Fluorescence Microscopy Image. (Used function: Auto Level, Low level, Pseudo Color, Merge Image)

Bottom on the left is merged image without improvement of image. And the bottom on the right is merged image after improvement of image. These images are shot by JNOPTIC AcquCAM Pro/G3 camera

 

3-2-1 Effect of high-contrast image with simple operation (Monochrome)

Improvement Function about Microscopy Image Monochrome. (Used function: Auto Level)

Effective improvement of image with simple operation. This image is shot by JNOPTIC Pro/S3 camera

 

3-2-2 Effect of high-contrast image with simple operation (Color)

Improvement Function about Microscopy Image. (Used function: Auto Level)

Effective improvement of image with simple operation. These images are shot by JNOPTIC AcquCAM Pro/G3 camera

 

3-3-1 Live Pseudo Color (for color camera)

When you get fluorescence images using color camera, if the unwanted channel was shown because of cross talk, you could remove this channel to take advantage of function of Live Pseudo.
Improvement Function about Fluorescence Microscopy Live Camera Image. (Used function: Live Pseudo Color)
 The extraction of wanted color information from color image (Remove BR on RGB source) These images are shot by JNOPTIC AcquCAM Pro/G3 camera

3-3-2 Live Pseudo Color (for Monochrome camera)

When you get fluorescence images using monochrome camera, you can raise efficiency of acquisition for fluorescence images to add similar false color to the color to be shown on the eyepiece in real time.
Improvement Function about Fluorescence Microscopy Live Monochrome Camera Image. (Used function: Live Pseudo Color)
 Improvement of usage environment for monochrome camera to add false color on the image. These images are shot by JNOPTIC AcquCAM Pro/S3 camera

4. JNO-MHU(Option Unit)

Available measuring height with additional unit, JNO-MHU

※ JNO-MHU is sold separately as optional unit.

Left: Focus on top of sample (Z-axis reset), Right: Focus on bottom of sample (Z-axis height measurement)

 < Measuring condition : over 20x objective, temp 20°C >

Responding Model Measurement value unit Measurable height Error of measuring 1000㎛ Recommended measuring height
CX 41 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
CKX 41 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
BX – FM 0.2 ㎛ -9.9 ~ 29.9㎜ Below ±20㎛ Below ± 2000㎛
BX 51/53 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛
MX 51 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛
MX 61L/61 0.1 ㎛ -9.9 ~ 29.9㎜ Below ±10㎛ Below ± 1000㎛

 

5. Reporting to Excel

 

Specification

Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats& Frame rate 1920×1200 RGB24@ 54 fps
1920×1200 Y800@ 162 fps
1920×1200 Y16@ 80 fps                             1920×1200 Y411 @ 108 fps
1920×1200 YUY2 @ 81 fps
Dynamic range 8 / 12 bit
Exposure time 20 ㎲ to 30s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65g

 

 

More images with AcquCAM 23GR2

-> For more details, click here.

 

 

AcquCAM 5G

AcquCAM 5G

 

(Down load Catalog – ACQUCAM 5G)

 

  • USB 3.0 Color Camera
  • 2/3 ” Pregius Sensor
  • 2448 x 2048 pixel
  • Trigger input and I/O
  • Acquisition of images with sufficient contrast
  • High Sensitivity Sensor
  • Fast, seamless image transfer
  • Available of images with a very wide field of view
  • Low Noise Sensor

 

 

AcquCAM 5G color camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The color camera ships with the very sensitive 2/3 inch CMOS Pregius  sensor. With up to 38 images per second, the AcuqCAM 5G is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses. Using the optional CS to M12 board lens adapter, the camera is
also compatible to M12 board lenses.

 

Images with AcquCAM 5G

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 5G
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

 

Specification

Spec Description
Size 2/3 inch
Resolution H: 2448, V: 2048
Pixel size H: 3.45 µm, V: 3.45 µm
Video formats& Frame rate 2448×2048 RGB64 @ 38 fps
2448×2048 RGB32 @ 35 fps
2448×2048 Y16 @ 37 fps                             2448×2048 Y411 @ 38 fps
2448×2048 YUY2 @ 37 fps
Dynamic range 8 / 12 bit
Exposure time 25 ㎲ to 4s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65g

 

ARM

ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.

-> For more details, click here.

 

More images with AcquCAM 5G

-> For more details, click here.

 

 

PE-300ultra

 

CoolLED pE-300ultra

 

NOW DOUBLE THE INTENSITY AT THE SAMPLE PLANE

CoolLED’s pE-300ultra is a fluorescence microscopy light source which offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains.  Users have access to both microsecond switching via multiple TTL inputs and the ability to mount inline excitation filters.  This, when paired with today’s high performance multi band filter sets, facilitates imaging traditionally done via a white light source and a filter wheel, with all the benefits of LED.

 

The pE-300ultra is the most controllable member of the pE-300 Series.  In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength intensity and shuttering.  Until now these benefits have only been accessible to users of high end, highly flexible illuminators such as the CoolLED pE-4000.

Throughout 2017 we will be releasing exciting new products and product updates.  The first member of the CoolLED product range to receive the star treatment is the pE-300 Series.

As part of the award winning pE-300 Series, the NEW Enhanced pE-300ultra  now delivers DOUBLE the intensity at the microscope sample plane.  It allows adjustment of output in 1% steps, giving precise control.  The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.

It is now possible to view and image your samples brighter than ever.

Convaleria taken with Photometrics Prime camera with 10ms exposure.

 

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. With the new enhanced pE-300ultra, all 3 channels at full power use just 46 Watts. That is almost a quadruple efficiency increase!

This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology.  Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

CoolLED’s pE-300 Series  was the 2017 winner of the “Go Beyond Award” in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities”  The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.

The pE-300ultra  offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light.  This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.

The system comprises a pE-300ultra Light Source, Control Pod, set of three Excitation Filter Holders and Power Supply.  It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

A benefit of the pE-300ultra is that  stains can be viewed either individually or in combination, without filter cube changes.  This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether. For further information on improved multi-stain testing, go to the Downloads tab.

An important additional feature of the pE-300ultra is that it includes CoolLED’s “Sequence Runner” multiple channel excitation mode.  Users can define the order of their fluorophore capture using their pE-300ultra Control Pod, then the pE-300ultra light source can accept a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels.  This feature is independent of the individual channel TTL inputs on the light source.  This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.

With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300ultra can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

 

 

There are two pE-300ultra configuration options:

Direct-fit for connecting to a microscopes – by selecting from a range of microscope adaptors  which covers all current and most older models.  A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guidewith a fixed 3mm diameter, liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

 

The pE-300ultra offers:

  • Clean bright illumination across the spectrum – excites common fluorescent stains
  • Specify for  existing single and multi-band filter sets – no need to buy new filters
  • Instant on/off – No shutters required, no warm up or cool down
  • Simple to fit, simple to use – no alignment, a once only adjustment
  • Stable & repeatable – reliable and consistent results
  • Wide range of microscope adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0-100%) – no ND filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time
  • Removable inline excitation filter holders – no moving parts
  • Individual channel triggering via TTL in microseconds
  • Sequence Runner – sequenced excitation from a single TTL-out

 

 

 

JNO-G(L)

JNOPTIC Filter JNO-G(L)

Download Catalog_JNO-G(L)

 

Filter set forAT – TRITC/Cy3 Longpass

  • Main Fluorochromes : TRITC
EX(nm) EM(nm)
555 580

 


Fluor VS JNO-G(L) Spectrum

 

TRITC VS JNO-G(L)

 


JNO-G(L) Spectrum

 

JNO-G(L) Spectrum
Filters Type
AT540/25x EX
AT565DC BS
AT575lp EM

 


JNO-G(L) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 


JNO-G(L) Image

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-G(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-G(L)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : UPLNAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-G(L)
Camera : AcquCAM 5G
C-mount adapter : 1X
Lens : UPLNAPO40X

 


Best with these fluorochromes

 

  • R-phycoerythrin

 


 

JNO-B(L)

JNOPTIC Filter JNO-B(L)

Download Catalog_JNO-B(L)

 

Filter set forAT – GFP/FITC Longpass

 

  • JNO-B(L) filter set induces the light of the Blue wavelength range to the sample and leads the generated fluorescence to the observation system.
  • Blue excitation (Longpass Emission)
  • 465~495nm, BS : 505nm, EM : 515nm

 


JNO-B(L) Spectrum

 

JNO-B(L) Spectrum
Filters Type
AT480/30x EX
 AT505DC BS
AT515lp EM

 


JNO-B(L) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

*** For filters larger than 25m and dichroics larger than 26x38mm, please contact us for pricing.

 


Fluorochromes

 

Main Fluorochromes FITC

 

 * FITC VS JNO-B(L)

 


JNO-B(L) Image

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-B(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-B(L)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : UPLNAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-B(L)
Camera : AcquCAM 5G
C-mount adapter : 1X
Lens : UPLNAPO40X

 


  • Acridine Orange + DNA and RNA
  • Auramine-rhodamine
  • JC-1 non-ratiometric

 


 

JNO-U(L)

JNOPTIC Filter  JNO-U(L)

Download Catalog_JNO-U(L)

 

Filter set forAT – UV/DAPI Longpass

 

  • JNO-U(L) filter set induces the light of the UV wavelength range to the sample and leads the generated fluorescence to the observation system.
  • UV excitation (Longpass Emission)
  • EX : 361~389nm, BS : 415nm, EM : 435nm

 


JNO-U(L) Spectrum

 

JNO-U(L) Spectrum
Filters Type
AT375/28x EX
AT415DC BS
AT435lp EM

JNO-U(L) Spec

 

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

*** For filters larger than 25m and dichroics larger than 26x38mm, please contact us for pricing.

 


Fluorochromes

 

Main Fluorochromes : DAPI

* DAPI VS JNO-U(L)

 


JNO-U(L) Image

 

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1X
Lens : UPLNAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : UPLNAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(L)
Camera : AcquCAM 5G
C-mount adapter : 1X
Lens : UPLNAPO40X

 


Best with these fluorochromes

 

  • Calcofluor White
  • DAPI

 


cell^TIRF

cell^TIRF

 

Cell^TIRF 소개

동시획득을 위한 OLYMPUS cell^TIRF Motorized Multicolor TIRFs

Objective-based TIRF microscopy 의 선도주자인 OLYMPUS는 multicolor TIRF 분야에서 중요한 도약을 발표하였습니다. 올림푸스 cell^TIRF 광원은 동시에 일어나는 이미지캡쳐를 위한 4개의 전동 채널들을 제공합니다. TIRF 매개변수들의 직관적인 소프트웨어 제어는 widefiled 형광과 TIRF 각도 및 끊김없는 이동의 즉각적인 설정과 확정을 가능하게 합니다. Cell^TIRF 광원으로 각 레이저파장은 최적으로 포커싱되고 각각의 각도는 개별적으로 설정, 다른 파장들이 같은 penetration depth를 가질수 있게 합니다. 사용자는 한번의 마우스 클릭으로 모든 레이져들의 계산된 penetration depths들을 미리 설정할 수 있습니다.
시스템은 개별적으로 모든 채널들로부터 TIRF 정보를 동시에 캡쳐하도록 각 레이저의 각도를 조절합니다.


특징 및 이득

Features and Benefits

● 유일한 진정한 동시 획득의 4 라인 시스템
● TIRF 각도의 전동 제어
● 정밀한 각도 조절
●  Penetration depth의 소프트웨어 계산
●  소프트웨어 기반의 레이저 감쇠 기능
●  첫번째 레이저 라인의 통합된 Point FRAP 광학계
●  끊김없는 widefield/TIRF 스위칭
●  NA 1.65 의 세계 최고의 6개의 전용 TIRF 렌즈
●  405 ~ 640nm 레이저 시스템
– 최적의 파장을 선택할 수 있는 유연성
●  340nm 투과의 TIRF 와 widefield 혹은 100% widefield
●  각 레이저 라인에 대한 25mm 필터 이용
●  각 레이저 라인에 대한 조절 가능한 Filed stop
●  단단한 단일 알루미늄 프레임
OLYMPUS cell^TIRF Publications
OLYMPUS cell^TIRF Gallery

Specifications

TIRF illuminator Available laser Systems 405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm
Laser coupling FC or FC8 connector
Field Number FN11.8
Illuminator Body Single Billet Aluminum
Safety Integrated Interlock
Laser systems Wavelengths 405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm
Power Laser system available up to 100mW
Attenuation Software control of laser intensity
Imaging Shutter High speed 1msec TTL shutters
Safety Integrated interlock
Epi-Fluorescence Light source 100W Mercury, 75W Xenon, Metal halide.
Selection TIRF/Brightfield or Brightfield only with 340nm transmission
Field number FN22
TIRF Objectives 60X Objectives
PLANAPON60XOTIRFM PLANAPO 60X, NA 1.45, WD 0.1mm, Correction collar
APON60XOTIRFM APO 60X, NA 1.49, WD 0.1mm, Correction collar
100X Objective
PLAPO100XOTIRFM PLANAPO 100X, NA 1.45 WD 0.10mm
UAPON100XTIRF UAPON 100X, NA 1.49 WD 0.09mm, Correction collar
AP0100XO-HR-SP APO 100X, NA 1.65, WD 0.10mm
150X Objectives
UAPON150XOTIRF UAPO 150X, NA 1.45,WD 0.07mm,Correction collar
Immersion Oil Type-F Low Auto Fluorescence Immersion oil
Coverslips High Refractive Index RI 1.78 Immersion oil for use with AP0100XO-HR-SP 1.65NA 100X objective
Compatible microscopes